Ulicny, K. J, D.R. Stewart, A. McElwain, E.R. Salmon, S.A. Bullard, J.O. Cook, G.B. Skomal, H.L. Pratt, Jr., and G.W. Benz. 2007. Sealing Whirl-Pak-type bags containing wet samples. Tennessee Academy of Science 88-89.
Abstract
Whirl-Pak and similar “fold-and-twist” blended polyethylene bags (FATs) are excellent containers for many types of biological samples because they are shatter-proof, lightweight, flat and thin when empty, less expensive than glass containers, nearly transparent, and available in a variety of sizes. Nevertheless, experience suggest that few know how to properly seal FAT’s, and this matter seems exacerbated by incorrect manufacturer instructions regarding the sealing of FAT’s containing wet samples. WE provide instructions to securely seal FAT’s containing wet samples which require: a sample without sharp structures that may puncture the FAT, proper FAT loading, proper FAT compression, and proper locking of the FAT seal. Executing the steps necessary to meet these requirements will produce a sealed FAT that can undergo subsequent changes in pressure and remain well-sealed for months to years and even if dropped.