Cooperative Fish and Wildlife Research Units Program: Arizona
Education, Research and Technical Assistance for Managing Our Natural Resources

Arizona Project


Can Ultrasonic Imaging Be Used in Remote Field Environments? A Case Study in Grand Canyon

June 2013 - July 2017


Personnel

Participating Agencies

  • USGS
Arizona Coop Unit student and USGS science staff setting hoop nets to capture fishes in the Grand Canyon of the Colorado River.

Ultrasonic imaging, also called ultrasound, is an effective, non-lethal method used to determine sex and maturity of a variety of freshwater, anadromous, and marine fishes. However, most previous studies have been performed in laboratory environments. We developed a standardized method for ultrasonically scanning endangered Humpback Chub Gila cypha in remote locations within Grand Canyon, Arizona, USA. This method minimized stress to individual fish and took less than 1 min to perform. We were able to identify female fish with eggs based on two jpeg images and one 10 s video clip collected in the field. We also used ImageJ®, a National Institute of Health image processing program, to develop a brightness index to evaluate the maturity of eggs in female fish. We collected ultrasonic scans of captive, ripe Humpback Chub held at the Southwestern Native Aquatic Resources and Recovery Center (SNARRC) to determine that female fish were potentially ripe when a subsample of their eggs exhibited a brightness value within the 32-44 range. Although we were able to estimate egg maturity, we were not able to estimate egg mass of female fish. We successfully scanned 751 Humpback Chub in the field and collected jpeg images and video clips for each fish. Fisheries managers can use this noninvasive technique in remote or rugged field locations to collect vital information about the reproductive status of fishes that cannot be killed. A thesis on this work was completed in May, 2016, and results are published in the North American Journal of Fisheries Management. Partners were the USGS Grand Canyon Monitoring and Research Center.

Presentations Presentation Date
Brizendine, M. E., D. L. Ward, S. A. Bonar, and W. J. Matter. 2016. Use of ultrasonic imaging to evaluate egg maturation of humpback chub Gila cypha in the Grand Canyon. 49th Joint Annual Meeting of the Arizona and New Mexico Chapters of The Wildlife Society and Arizona/New Mexico Chapter of The American Fisheries Society, February 4-6, 2016, Flagstaff, Arizona 2016-02-05
Brizendine, M. E., D. L. Ward and S. A. Bonar. 2013 Use of ultrasonic imaging and Ovaprim® to evaluate egg maturation of humpback chub, Gila cypha. 45th Annual Meeting of the Desert Fishes Council. November 20-24, Flagstaff, Arizona. 2013-11-24
Brizendine, M. E., D. L. Ward, S. A. Bonar, and W. J. Matter. 2015 ,Use of ultrasonic imaging to evaluate egg maturation of Humpback Chub, Gila cypha, in Grand Canyon 47th Annual Meeting 18-22 November 2015 Death Valley National Park, California, U.S.A. 2015-11-20
Brizendine, M. E., S. A. Bonar, D. L. Ward and W. J. Matter. 2014. Use of ultrasonic imaging and Ovaprim® to evaluate egg maturation of Humpback Chub. 47th Joint Annual Meeting of the AZ and NM Chapters of the American Fisheries Society and The Wildlife Society, Pinetop Arizona, February 6-8, 2014. 2014-02-07
Brizendine, M., D. Ward, S. A. Bonar, and W. Matter. 2015. Use of Ultrasonic Imaging to Evaluate Egg Maturation of Humpback Chub Gila cypha in the Grand Canyon. 145th Annual Meeting of the American Fisheries Society, Portland, Oregon. 2015-08-17
Brizendine, M., S. A. Bonar, and D. Ward. 2013. Evaluation of Humpback Chub Gila cypha egg maturation using ultrasonic imaging and Ovaprim®. 143nd Annual Meeting of the American Fisheries Society, Little Rock, Arkansas, September 8-12, 2013. POSTER PRESENTATION. 2013-09-08
Brizendine, M.E., D.L. Ward, S.A. Bonar, and W.J. Matter. 2015. Use of ultrasonic imaging to evaluate egg maturation of humpback chub Gila cypha. The Joint Annual Meeting of the Arizona/New Mexico American Fisheries Society and the Wildlife Society, Las Cruces, New Mexico. February 5 – February 7, 2015. Contributed Oral Presentation. 2015-02-07
Brizendine, M.E., S. A. Bonar, D. L. Ward, and W. J. Matter. 2014. Use of ultrasonic imaging and Ovaprim® to evaluate egg maturation of humpback chub Gila cypha. The Western Division of the American Fisheries Society Annual Meeting, Mazatlán, Mexico. April 7 – April 11, 2014 2014-04-08
Theses and Dissertations Publication Date
Brizendine, M. E. 2016. Use of ultrasonic imaging to evaluate egg maturation of humpback chub Gila cypha. Master's Thesis, University of Arizona, Tucson. 2016-05-31